Future of Biological Aerosol Detection


  1. Ho, J.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Biological aerosol detection in real time is an urgent civilian and military requirement. Such detection capability will be useful in environmental monitoring, for example, in gathering information in perceived hazardous areas such as housing developments downwind of sewage treatment plants. To be truly functional, the instrument has to operate continuously, 24 hours a day and 7 days a week with minimal maintenance and few false alarms. A novel concept is proposed. The system employs a rapid front-end warning/alarming mechanism based on optical technologies that provides useful information for protection decision makers. This is connected to a sample collector that feeds a slower back-end liquid chemistry system that provides analytical results to the medical personnel to assist in prophylaxis and therapy decisions. Experience gained from measuring fluorescence signals of single bacterial spores under flow cytometry (FCM) using UV excitation at 340-360 nm, was applied to concept testing of a prototype instrument, built to do the same for aerosols. This machine was capable of resolving particle size as well as fluorescence intensity of each particle under laboratory and field conditions; it was called the fluorescent aerodynamic particle sizer (FLAPS). TRUNCATED
Fluorescence in situ hybridization;Antigen masking;Flow cytometry;Bio-aerosol detection;Fluorescence of Biological Aerosols;FLAPS (Fluorescent Aerodynamic Particle Sizer)
Report Number
DRDC-SUFFIELD-SL-2001-113 — Reprint
Date of publication
27 Aug 2002
Number of Pages
Reprinted from
Analytica Chimica Acta, vol 457, 2002, p 125-148
Hardcopy;Document Image stored on Optical Disk

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