MENINGOCOCCAL CONTROL IN THE CANADIAN FORCES. III. HANDLING PROCEDURES TO ENSURE MAXIMAL RECOVERY OF NEISSERIA MENINGITIDIS FROM AIR SAMPLES

Authors
  1. Holbein, B.E.
  2. Spence, M.R.
  3. White, L.A.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Representative strains of Neisseria meningitidis were found to be sensitive to holding in Trypticase Soy Broth (TSB) at 20C, after aerosolization and collection. The viability losses observed during holding were suggestive of aerosolization-induced damage. The strains of N. meningitidis examined were also found to be sensitive to freezing and thawing. Of several cryoprotective agents examined, only dimethyl sulphoxide (DMSO) at concentrations between 5 and 10% was found to provide protection during freezing. It was concluded that air samples suspected of containing N. meningitidis should be plated for assessment within 10 minutes, or treated with DMSO to a concentration between 5 and 10%, and frozen on dry ice, within 10 minutes.
Report Number
TN-412 —
Date of publication
15 Oct 1977
Number of Pages
13
DSTKIM No
77-04722
CANDIS No
101394
Format(s):
Hardcopy;Originator's fiche received by DSIS

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