NONSPECIFIC INTERACTION OF STREPTAVIDIN WITH UREASE-CONJUGATED ANTIBODIES

Authors
  1. Thompson, H.G.
  2. Lee, W.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Streptavidin (SA) biotin interactions are used in many aspects of bioanalysis and molecular biology. The biosensor of the Bio-Chemical Detector (BCD) employs SA biotin-mediated filtration-capture onto nitocellulose membranes in its immunoassay procedures. During the development of immunoassays for the BCD biosensor, systematic variations in the background and the sensitivity were observed. The results presented in this work indicated that the cause of these variations was nonspecific interaction of SA with one of the components of the reagent mixture, a urease-conjugated antibody. The variations could be diminished by allowing the reagents to stand at room temperature for two to three hours prior to use.
Report Number
DRES-M-1358 — Memorandum
Date of publication
15 Nov 1991
Number of Pages
25
DSTKIM No
92-00443
CANDIS No
103341
Format(s):
Hardcopy;Originator's fiche received by DSIS

Permanent link

Document 1 of 1

Date modified: