RAPID IMMUNOFILTRATION ASSAY OF FRANCISELLA TULARENSIS

Authors
  1. Thompson, H.G.
  2. Lee, W.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A rapid immunofiltration assay for a killed bacterial preparation of Francisella tularensis was developed. Formalin treated cells of Francisella tularensis were incubated with polyclonal antibody to Francisella tularensis and an anti-species antibody conjugated to urease. The reaction mixture was filtered through albumin coated 0.45 mu m nitrocellulose membranes following incubation periods which varied from 1 to 60 min. The pH sensing capability of the detector, a light-addressable potentiometric (LAP) sensor, was employed to detect the presence of immobilized urease-conjugated antibodies. The time required for the assay was about 5 minutes exclusive of the incubation period. Limits of detection (LOD) of the assay, determined as a function of the incubation time were 1.2 x E5, 5 x E4, and 3.4 x E3 cells for incubations of 1, 5, and 60 minutes. The speed and ease of the assays suggests that the LAP sensor technology is suitable as a detection system for a mobile field laboratory.
Report Number
DRES-M-1376 — Memorandum
Date of publication
15 Mar 1992
Number of Pages
25
DSTKIM No
92-01363
CANDIS No
104254
Format(s):
Hardcopy;Originator's fiche received by DSIS

Permanent link

Document 1 of 1

Date modified: