DETECTION AND IDENTIFICATION OF HUMAN INFLUENZA VIRUSES BY THE POLYMERASE CHAIN REACTION

Authors
  1. Evans, D.H.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Guelph Univ, Guelph ONT (CAN) Dept of Molecular Biology and Genetics
Abstract
The experimental component of SSC contract W7702-8-R077/01-XSG was intended to investigate whether oligonucleotide primers designed to hybridize to conserved regions of influenza viruses could be used to detect and amplify these viruses using the polymerase chain reaction. The goal of this project (FAX from Mr. D. Pickles, 20 June 1989) was to try and develop probes specific for Orthomyxoviruses as a group and, if possible, differentitate influenza A and B strains. We found that the sequences of influenza A, B, and C strains are simply too divergent to permit the construction of a single set of "generic" influenza probes. However, within strains there are sufficiently conserved regions of sequence to permit the construction of A-specific, B-specific, and C-specific probes. These primers appear to fulfill the expectations of the contract since in combination they can probably detect most influenza strains likely to infect humans. TRUNCATED
Report Number
DRES-CR-21-92 — Contract Report (Final)
Date of publication
15 Mar 1991
Number of Pages
57
DSTKIM No
92-01586
CANDIS No
104476
Format(s):
Hardcopy;Originator's fiche received by DSIS

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