RAPID ESTIMATION OF POST EXPOSURE INCAPACITATION: 1. DEFINITION OF THE ROTOPAD AND SPASM TESTS

Authors
  1. Bide, R.W.
  2. Risk, D.J.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A simple test to indicate impaired motor function in mice is described. The test can be completed within 2 min using naive mice. Training of the animals is not a requisite. In normal mice, 94.7% of the population pass the test. Impaired motor function (Significant P < 0.01) is indicated when 2 of 5 animals fail the test. The effects of sodium pentobarbital, diazepam and chlorpromazine were studied to validate the procedure. All three drugs produced impaired function. Halothane anaesthesia (by inhalation exposure) was also tested but the recovery of the mice was too fast for reliable estimates of impaired motor function to be obtained. A second test is described in which the tonic convulsions that occur spontaneously in CD-1 mice when they are suspended by the tail are used as a measure of seizure activity. Enhancement and suppression of the convulsions can be demonstrated. TRUNCATED
Report Number
DRES-M-1372 — Memorandum
Date of publication
15 Mar 1992
Number of Pages
31
DSTKIM No
92-01883
CANDIS No
104768
Format(s):
Hardcopy;Originator's fiche received by DSIS

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