BIOSENSOR-BASED DETECTION OF SOMAN

Authors
  1. Lee, W.E.
  2. Hall, J.G.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
An enzymatic assay, using a silicon-based light addressable potentiometric (LAP) sensor, was developed for aqueous samples containing the organophosphorus anticholinesterase, soman. The method entailed titration of biotinylated acetylcholinesterase with soman, followed by filtration-capture immobilization onto nitrocellulose membrane via streptavidin-biotin interactions. Total assay time was about 12 - 15 min and a lower limit of detection of about 2 pg of soman was obtained. Previously the LAP sensor has been shown to be an effective detection system for protein, DNA, virus and bacteria. This work has demonstrated that, in addition to biological materials, the LAP sensor may provide very good sensitivity toward anticholinesterase compounds.
Report Number
DRES-M-1380 — Memorandum
Date of publication
15 Apr 1992
Number of Pages
21
DSTKIM No
92-01886
CANDIS No
104771
Format(s):
Hardcopy;Originator's fiche received by DSIS

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