IMMUNOCHEMICAL IDENTIFICATION OF NEWCASTLE DISEASE VIRUS STRUCTURAL PROTEINS

Authors
  1. Fulton, R.E.
  2. Shew, R.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The immunochemical reactivity of the structural polypeptides of two strains of Newcastle Disease Virus (La Sota and B1), when reacted with hyperimmune polyclonal antisera, has been studied. Methods are described for the separation of NDV proteins by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (reduced and non-reduced, the electrophoretic transfer of separated proteins to nitrocellulose paper, and the subsequent identification of NDV antigenic determinants by a sensitive enzyme-linked immunosorbent assay. Using these methods, a number of new, minor polypeptides have been detected which hitherto have not been described.
Report Number
378 —
Date of publication
15 Jan 1984
Number of Pages
24
DSTKIM No
84-01357
CANDIS No
120794
Format(s):
Microfiche filmed at DSIS;Originator's fiche received by DSIS;Hardcopy

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