THE USE OF 4'-AMINOMETHYL-4,5',8-TRIMETHYLPSORALEN IN THE INACTIVATION OF INFLUENZA A/PR8

Authors
  1. Toenjes, S.J.
  2. Fulton, R.E.
  3. Mofford, L.M.
  4. and others
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A procedure which utilizes the property of 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) to bind to single stranded RNA in the presence of long wave ultraviolet light (LWUV) has been developed for the inactivation of influenza A/PR8. At a concentration of AMT of 10 mu g/ml, inactivation was complete after an exposure time to LWUV of three min. Virus infectivity was not significantly affected by treatment with either AMT or LWUV alone. The ability of influenza A/PR8 to haemagglutinate rooster erythrocytes was unaffected by AMT-LWUV treatment. The procedure provides an alternative to chemical inactivation procedures which can alter the antigenicity of virus preparations and thereby compromise their value as protective immunogens.
Report Number
376 —
Date of publication
15 Jan 1984
Number of Pages
29
DSTKIM No
84-01359
CANDIS No
120796
Format(s):
Hardcopy;Originator's fiche received by DSIS

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