BIOMOLECULAR ANALYSIS AND EVALUATION OF LIPOSOMAL CARRIERS

Authors
  1. Stanacev, N.Z.
Corporate Authors
Toronto Univ, Toronto ONT (CAN) Dept of Clinical Biochemistry;Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
Liposomes have been shown to be very effective in enhancing the immunogenicity of feeble protein antigens. The relationship of lipid-protein interactions and the potentiated immune response, however, is not well understood. In this study, the molecular distribution of antigenic molecules in liposomes was determined and correlated with the engendered immune enhancement. The topography of a protein antigen, bovine serum albumin (BSA), associated with different liposome preparations was analyzed by electron spin resonance method. Both hydrophobic spin labeled lecithin and hydrophilic spin marker, Tempocholine, were used; a spin labeled BSA was also prepared. Addition of L-ascorbate to these vesicles resulted in the loss of spin labels present on the exterior surface. TRUNCATED
Date of publication
15 Jul 1984
Number of Pages
73
DSTKIM No
84-02972
CANDIS No
122250
Format(s):
Microfiche filmed at DSIS;Originator's fiche received by DSIS;Hardcopy

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