USE OF KANAMYCIN FOR THE ISOLATION OF AUXOTROPHIC MUTANTS OF ACTINOBACILLUS MALLEI

Authors
  1. Evans, D.H.
Corporate Authors
Defence Chemical Biological and Radiation Labs, Ottawa ONT (CAN)
Abstract
Growth of Actinobacillus mallei was inhibited by kanamycin. Higher concentrations had a pronounced bactericidal effect. Cells irradiated with ultraviolet light were grown in media, washed, and exposed to kanamycin. Auxotrophic mutants with requirements for tryptophane, phenylalanine, proline, and uracil were detected. After treatment with nitrous acid and growth in medium supplemented with amino acids, cells were washed and exposed to kanamycin. The proportion of auxotrophs among the survivors varied from 1.3 to 75%. Mutants with requirements for amino acids were identified, with methionine-requiring mutants predominating. Exposure of mixtures of prototrophs and uracil-dependent and methionine-dependent auxotrophs to kanamycin resulted in approximately 700- and 300-fold increases, respectively, in the ratio of auxotrophs to prototrophs.
Report Number
DCBRL-494 — Reprint; Previously accessioned as DSIS 66-14389
Date of publication
01 Jan 1966
Number of Pages
13
Reprinted from
Canadian J of Microbiology, vol 12, 1966, p 641-652
DSTKIM No
92-03516
CANDIS No
126885
Format(s):
Hardcopy;Originator's fiche received by DSIS

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