USE OF KANAMYCIN FOR THE ISOLATION OF AUXOTROPHIC MUTANTS OF ACTINOBACILLUS MALLEI
- Authors
- Corporate Authors
- Defence Chemical Biological and Radiation Labs, Ottawa ONT (CAN)
- Abstract
- Growth of Actinobacillus mallei was inhibited by kanamycin. Higher concentrations had a pronounced bactericidal effect. Cells irradiated with ultraviolet light were grown in media, washed, and exposed to kanamycin. Auxotrophic mutants with requirements for tryptophane, phenylalanine, proline, and uracil were detected. After treatment with nitrous acid and growth in medium supplemented with amino acids, cells were washed and exposed to kanamycin. The proportion of auxotrophs among the survivors varied from 1.3 to 75%. Mutants with requirements for amino acids were identified, with methionine-requiring mutants predominating. Exposure of mixtures of prototrophs and uracil-dependent and methionine-dependent auxotrophs to kanamycin resulted in approximately 700- and 300-fold increases, respectively, in the ratio of auxotrophs to prototrophs.
- Report Number
- DCBRL-494 — Reprint; Previously accessioned as DSIS 66-14389
- Date of publication
- 01 Jan 1966
- Number of Pages
- 13
- Reprinted from
- Canadian J of Microbiology, vol 12, 1966, p 641-652
- DSTKIM No
- 92-03516
- CANDIS No
- 126885
- Format(s):
- Hardcopy;Originator's fiche received by DSIS
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