THE EFFECT OF LETHAL DOSES OF X-IRRADIATION ON THE ENZYMATIC ACTIVITY OF MITOCHONDRIAL CYTOCHROME c

Authors
  1. Scaife, J.F.
Corporate Authors
Defence Chemical Biological and Radiation Labs, Ottawa ONT (CAN)
Abstract
The coupling of the tetrazolium salts to the electron transport chain in mitochondria of thymus, spleen, liver, kidney, and Ehrlich ascites carcinoma cells has been studied with several substrates. It has ben possible to demonstrate a radiation lesion in the electron transport chain of mitochondria from thymus and spleen, but not in those from other tissues. This lesion is evident 4 hours after 25 rad of whole-body irradiation, or earlier with higher doses. It is not prevented by the prior administration of aminoethylisothiouronium bromide, serotonin, vitamin K1 or vitamin E, but is reduced by anoxic conditions. The radiation lesion is believed to be produced by loosening the binding of cytochrome c to the mitochondrial membrane after X-irradiation.
Report Number
DCBRL-478 — Reprint; Previously accessioned as DSIS 66-06399
Date of publication
15 Sep 1965
Number of Pages
17
Reprinted from
Canadian J of Biochemistry, vol 44, 1966, p 433-448
DSTKIM No
92-03622
CANDIS No
126981
Format(s):
Hardcopy;Originator's fiche received by DSIS

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