GROWTH OF ACTINOBACILLUS MALLEI IN CHEMICALLY DEFINED MEDIA

Authors
  1. Evans, D.H.
Corporate Authors
Defence Chemical Biological and Radiation Labs, Ottawa ONT (CAN)
Abstract
A simple, chemically defined liquid medium containing phosphate buffer, magnesium and ferrous sulfates, glucose, and tribasic ammonium citrate supported excellent growth of a virulent strain of Actinobacillus mallei. When growth was measured turbidimetrically, a comparison of the rates of growth in media containing different initial concentrations of hydrogen ion (pH 6.3 and 6.6), phosphate buffer (0.05 M and 0.08 M), glucose (0.05 M and 0.10 m), and ammonium citrate (0.01 M and 0.02 M) indicated that the lower concentration of phosphate, glucose, and citrate and pH 6.3 favored early growth. The higher concentration of glucose favored an increase in cell mass during the early stationary phase unaccompanied by a comparable increase in viable cells.
Report Number
DCBRL-493 — Reprint; Previously accessioned as DSIS 66-14375
Date of publication
15 Jun 1965
Number of Pages
8
Reprinted from
Canadian J of Microbiology, vol 12, 1966, p 617-623
DSTKIM No
92-03872
CANDIS No
127199
Format(s):
Hardcopy;Originator's fiche received by DSIS

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