PURIFICATION AND PARTIAL CHARACTERIZATION OF MONOCLONAL ANTIBODIES OF FRANCISELLA TULARENSIS

Authors
  1. Bhatti, A.R.
  2. Wong, J.P.
  3. Woods, D.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Hybridomas secreting monoclonal antibodies against Fancisella tularensis cellular antigens were isolated. These monoclonal antibodies reacted with F. tularensis in ELISA but not by immunoblot, indicating that the antibodies are directed against conformational epitopes. One of these monoclonal antibodies was directed principally against outer membrane protein (OMP) components, and the remainder are likely directed against capsular components. The OMP-specific monoclonal antibodies are F. tularensis-specific in contrast to the others which cross-react with a number of other bacterial species. These OMP-specific monoclonal antibodies are of the IgG sub 1 subclass, and may prove to be a useful tool for diagnosis of F. tularensis in clinical materials.
Keywords
Francisella tularensis
Report Number
DRES-M-1383 — Memorandum
Date of publication
15 Jul 1992
Number of Pages
23
DSTKIM No
92-03879
CANDIS No
127206
Format(s):
Hardcopy;Originator's fiche received by DSIS

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