LIPSOMES AND BRONCHOALVEOLAR LAVAGE FLUID: RELEASE OF VESICLE-ENTRAPPED GLUTATHIONE

Authors
  1. Jurima-Romet, M.
  2. Barber, R.F.
  3. Shek, P.N.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN);Banting Research Centre, Ottawa ONT (CAN) Life Sciences Div
Abstract
Interest in the potential applications of liposomes for pulmonary drug delivery prompted this in vitro study of the effect of lipid composition on vesicle permeability. Rat cell-free bronchoalveolar lavage fluid was incubated at 37C with large unilamellar liposomes, prepared by the extrusion technique, containing entrapped 3H-glutathione (GSH). Lavage-induced GSH leakage occurred for liposomes prepared from dipalmitoylphosphatidylcholine (DPPC). However, leakage from dimyristoylphosphatidylcholine (DPMC) in the presence of lavage was not different from leakage in control buffer. The effect of cholesterol was found to enhance GSH release from DSPC and DPPC vesicles, and to stabilize DMPC vesicles. The results suggest that DPPC liposomes may be more suitable than vesicles prepared from DSPC or DMPC for effective drug delivery to the lung, and that variation of cholesterol content can be used to selectively enhance or diminish release characteristics.
Report Number
DCIEM-92-P-63 — Research Paper; Technical Note; Reprint
Date of publication
10 Oct 1990
Number of Pages
10
Reprinted from
International J of Pharmaceutics, vol 88, 1992, p 201-210
DSTKIM No
93-00311
CANDIS No
127636
Format(s):
Hardcopy;Originator's fiche received by DSIS

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