CHARACTERISTICS OF THE BIOCHEMICAL DETECTOR SENSOR

Authors
  1. Lee, W.E.
  2. Jacobson, T.V.
  3. Thompson, H.G.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The biosensor module of the Biochemical Detector (BCD) concept model is composed of two major subsystems, a light-addressable potentiometric (LAP) sensor and a fluidic processor. The biosensor module is automated to allow for uptake, mixing, and incubation of reagents and sample, filtration of the incubated mixture of reagents and sample through a nitrocellulose membrane, and detection fo the membrane-immobilized analyte complexes by means of the LAP sensor. This report describes a series of experiments that were undertaken to compare the performance of the concept model with that of a commercial LAP sensor unit and an earlier breadboard version of the biosensor module of the BCS. Although the performance of the LAP sensor itself was essentially the same in all three units, it was found that for immunoassays which required mixing and incubation of reagents and sample, the output signals of the LAP sensor were generally lower on the concept model and breadboard than on the commercial unit. The differences in response are attributed to insufficient mixing of reagents and sample in the fluidic processors. The result of this work indicates that the current LAP sensor subsystem produces output signals of acceptable magnitude for well mixed reagents, but that the extent of mixing or reagent and sample by the fluidics processor is insufficient for the rapid immunoassays required of the BCD.
Keywords
LAP (Light Addressable Potentiometry);Biotin;Streptevidin
Report Number
DRES-M-1402 — Memorandum
Date of publication
01 Mar 1993
Number of Pages
31
DSTKIM No
93-02301
CANDIS No
130081
Format(s):
Hardcopy;Originator's fiche received by DSIS

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