PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES (MAB) TO FRANCISELLA TULARENSIS

Authors
  1. Schryvers, A.B.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Monoclonal antibodies (mAbs) against Francisella tularensis were originally developed at DRES primarily for potential use in the Biochemical Detector Program, although antibodies with immunotherapeutic potential were also considered of value. The original hybridoma lines had been stored frozen for a period of over 5 years and there were concerns regarding the viability of the cell lines and the stability of antibody production. At a first step to investigate the utility of anti-F. tularensis monoclonal antibodies (mAbs), the hybridoma lines were tested for viability and production of antibody in a previous short term contract. The current contract was initiated as an extension of this work in order to more extensively evaluate the mAbs produced by these hybridoma lines and to prepare additional hybridoma lines producing anti-F. tularensis mAbs in the event that the preexisting mAbs were not adequate. In addition, we initiated studies to evaluate alternate means of preserving useful antibodies that were identified.
Keywords
Francisella tularensis
Report Number
DRES-CR-93-29 — Contract Report (Final)
Date of publication
29 Apr 1993
Number of Pages
7
DSTKIM No
93-04607
CANDIS No
135343
Format(s):
Hardcopy;Originator's fiche received by DSIS

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