DETECTION OF BW AGENTS: FLOW CYTOMETRY MEASUREMENT OF BACILLUS SUBTILLIS (BG) SPORE FLUORESCENCE

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Authors
  1. Ho, J.
  2. Fisher, G.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The aim of the report is to demonstrate that flow cytometry (FC), a tool originally designed for mammalian cell studies, can be used to characterise micron size particles such as bacteria and spores. A commercial instrument optmised for microorganisms has been used to measure light scattering and fluorescent signals emitted from a single spore. The results show that exciting a spore with 365 nm light can provide three sets of information related to some particle characteristics. There is indirect evidence to suggest that the fluorescence signal is related to intra-spore biomolecules. Fluorescence could be a useful measurement for rapid non-specific detection of biological agents, functioning as a cueing system that can quantify and qualifyu collected particles as biological or non-biological. This detection scheme could help reduce potential false alarms in particle analysis systems as well as offering the distinct advantage of not requiring prerequisite antigenic knowledge of the threat agents. In addition, the technique requires no expenditure of costly reagents to operate and thus, is amenable to incorporation into continuous monitoring operations. It is also demonstrated that FC may be capable of identifying BW agents if specific antibodies are available. It is envisioned that this function can be activated after fluorescence measurements indicate a potential biological threat is present. TRUNCATED
Report Number
DRES-M-1421 — Memorandum
Date of publication
01 Oct 1993
Number of Pages
42
DSTKIM No
93-04950
CANDIS No
135828
Format(s):
Hardcopy;Originator's fiche received by DSIS;Document Image stored on Optical Disk

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