FIBRE OPTIC BIOSENSOR ASSAY OF NEWCASTLE DISEASE VIRUS

Authors
  1. Lee, W.E.
  2. Thompson, H.G.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A fluorometric sandwich immunoassay for Newcastle disease virus (NUV) was developed using a fibre optic biosensor. Antibodies directed against NDV were covalently attached to the surface of silane-coated quartz fibres and were used to capture the antigen onto the optical element. Fluorescein-labelled anti-NDV served as the detector antibody. Assay times were approximately 10 min in duration with a limit of detection of 5 ng of purified virus. The biosensor detection system possesses several desirable characteristics such as a limited number of mechanical components, a reusable solid support and low baseline variation. These and other properties of the fibre optic biosensor indicate that it has the potential to serve as an on-line continuous monitoring device of an automated or semiautomated detection system. As a part of the fibre optic biosensor development, the surface coverage of the quartz fibres by capture antibody was characterized and an enzyme immunoassay for NDV which employed quartz fibres as the solid support was undertaken. This work was conducted as part of a trinational (UK/US/CA) effort on the Biochemical Detector.
Report Number
DRES-580 —
Date of publication
01 Oct 1993
Number of Pages
44
DSTKIM No
94-00041
CANDIS No
135829
Format(s):
Hardcopy;Originator's fiche received by DSIS

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