INCORPORATION OF ALPHA-TOCOPHEROL IN LIPOSOMES PROMOTES THE RETENTION OF LIPOSOME-ENCAPSULATED GLUTATHIONE IN THE RAT LUNG

Authors
  1. Suntres, Z.E.
  2. Shek, P.N.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
The present study was undertaken to investigate whether alpha-tocopherol incorporated in liposomes could improve the retention of entrapped glutathione (GSH) in the lung following intratracheal instillation in rats. Rats were treated with a single dose of [3H]GSH entrapped in liposomes containing 30 or 0 mol% alpha-tocopherol and killed 0, 24 or 48 h later. The retention of GSH in the lung was assessed by measuring the recovery of either [3H] label or GSH content in the lung. Animals instilled with free [3H]GSH were found to retain only 2% of the administered dose by 24 h after treatment and no detectable radioactivity by 49 h. Liposome encapsulation altered the pulmonary retention of GSH with 18 and 10% of radioacitivity remaining in the lung at 24 and 48 h post treatment, respectively. The instillation of GSH encapsulated in alpha-tocopherol-containing liposomes resulted in the highest level of GSH retention in the lung, namely 37 and 30% of administered GSH dose at 24 and 48 h, respectively. Results from Sepharose 4B column chromatography revealed that lung homogenates, obtained from rats instilled with GSH entrapped in alpha-tocopherol-containing liposomes, 24 and 48 h earlier, contained 2 eluted GSH peaks - one associated with the liposomal lipid marker in the void volume and the other as free GSH tripeptide, suggesting a slow sustained release effect mediated by the liposomal fromulation. TRUNCATED
Report Number
DCIEM-92-P-56 — Research Paper; Reprint
Date of publication
09 Dec 1992
Number of Pages
6
Reprinted from
J Pharm Pharmacol, vol 46, 1994, p 23-28
DSTKIM No
94-01666
CANDIS No
140247
Format(s):
Hardcopy;Originator's fiche received by DSIS

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