IMMUNOASSAY OF NEWCASTLE DISEASE VIRUS AND ANTI-NDV IN MOUSE SERUM USING A LIGHT-ADDRESSABLE POTENTIOMETRIC SENSOR

Authors
  1. Wikjord, B.
  2. Thompson, H.G.
  3. Lee, W.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A rapid non-radioactive sandwich immunoassay which utilizes biotin-streptavidin mediated filtration capture of immune complexes in conjunction with a silicon sensor was developed for the detection of virus and antibody to virus in serum. Using Newcastle Disease Virus (NDV) and mouse anti-NDV IgG spiked into mouse serum as a clinical-style model, the lower limits of detection (LOD) were determined. The LODs per test site were 2 ng for NDV and 4 ng for anti-NDV. The assays were carried out at room temperature in a single step 60 min incubation of immunoreagents with analyte. The assays were easy to perform and required a total time equal to the incubation period plus about ten minutes. The assay format is suitable for a wide range of biological antigens. New assays can be developed and optimized readily, often within one day. The apparatus employs a light-addressable potentiometric (LAP) sensor, is rugged and compact, and requires minimal logistic support. The work presented here demonstrates the utility of the LAP sensor for use in a field or remote hospital setting.
Keywords
LAP (Light Addressable Potentiometry);Biotin;Streptavidin
Report Number
DRES-M-1423 —
Date of publication
01 Feb 1994
Number of Pages
24
DSTKIM No
94-02910
CANDIS No
141347
Format(s):
Hardcopy;Originator's fiche received by DSIS

Permanent link

Document 1 of 1

Date modified: