RAPID IMMUNOFILTRATION ASSAY OF NEWCASTLE DISEASE VIRUS USING A SILICON SENSOR

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Authors
  1. Lee, W.E.
  2. Thompson, H.G.
  3. Hall, J.G.
  4. Fulton, R.E.
  5. Wong, J.P.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Victoria Univ, Victoria BC (CAN) Dept of Biochemistry
Abstract
A rapid nonradioactive sandwich immunoassay which utilizes biotin-streptavidin mediated filtration capture of immune complexes in conjunction with a silicon sensor was developed for the detection of virus. Using purified Newcastle disease virus as a model, the lower limits of detection (LOD) were determined for a number of immunoassay configurations employing both monoclonal and polyclonal antibodies. The LODs ranged from 1.3 ng/ml (sample volume of 100 mu (m) 1) for an incubation of 60 min to 400 ng/ml for a 1 min incubation. The sandwich immune complexes were formed from one-step incubation of antibody and antigen. No 'hook' effects were observed over a wide range of analyte concentrations. The assays were easy to perform and required a total time equal to the incubation period plus about 5 min. The assay format is suitable for virus, bacteria and protein antigens. New assays can be developed and optimized readil, often within 1 day.
Keywords
Biotin;Streptavidin;Light addressable potentiometric sensor
Date of publication
02 Jul 1993
Number of Pages
11
Translation of
J of Immunological Methods, vol 166, 1993, p 123-131
DSTKIM No
95-00928
CANDIS No
144044
Format(s):
Hardcopy;Document Image stored on Optical Disk

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