HIGH SENSITIVE FLUOROGENIC ENZYME-LINKED IMMUNOSORBENT ASSAY: DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B1

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Authors
  1. Bhatti, A.R.
  2. Siddiqui, Y.M.
  3. Micusan, V.V.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A highly sensitive, indirect double sandwich fluorogenic enzyme-linked immunosorbent assy (FELISA), has been developed for the detection of staphylococcal enterotoxin B (SEB). The described FELISA allows a SEB quantification of 0.1 fg/ml for purified toxin and a detection limit of at least 10 pg/g of contaminated food. Given the sensitivity of FELISA elaborate procedures for the extraction and concentration of enterotoxin from food samples became unnessary. The monoclonal anti-SEB antibody (MCA a-SEB) used in the described method reacted with SEB only. The FELISA is simple to perform and, provided the reaction plates are pre-prepared, the results can be obtained in approximately 3 h.
Keywords
FELISA;Staphylococcal enetrotoxins
Date of publication
08 Sep 1993
Number of Pages
12
Reprinted from
Journal of Microbiological Methods, no 19, 1994, p 179-187
DSTKIM No
95-00877
CANDIS No
144045
Format(s):
Hardcopy;Document Image stored on Optical Disk

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