OPTIMIZATION OF A PHAGOCYTE MICROPLATE CHEMILUMINESCENT ASSAY

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Authors
  1. Kournikakis, B.
  2. Simpson, M.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Chemiluminescent assays have been used to quantify phagocytic activity since 1972. In recent years these assays have been adapted to the 96-well microplate format as new luminometers have been developed. In this report we describe the optimization of a lucigenin enhanced phagocyte chemiluminescent assay using a Titertek Luminoskan. Factors such as cell concentration, serum concentration in the opsonization of the zymosan used and lucigenin concentration were all optimized in our assay. In addition we have found that some of the unique features of the Luminoskan, continuous microplate agitation during the assay and microplate temperature control up to 43C, also significantly enhanced the chemiluminescent response.
Keywords
Titertek Luminoskan;Microplates;Luminometers;Lucigenin;Zymosan;Macrophages;Neutrophils
Date of publication
05 Apr 1994
Number of Pages
5
Reprinted from
J Biolumin Chemilumin, no 10, 1995, p 63-67
DSTKIM No
95-02449
CANDIS No
151453
Format(s):
Document Image stored on Optical Disk;Hardcopy

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