CAPILLARY ZONE ELECTROPHORESIS ANALYSIS AND DETECTION OF MID-SPECTRUM BIOLOGICAL WARFARE AGENTS

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Authors
  1. Boulet, C.A.
  2. Townsley, C.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
DRE Suffield has initiated a research program to develop methods and equipment for field detection and laboratory identification of mid-spectrum agents, molecules of biological origin such as proteins, peptides and toxins. In this study, a highly efficient and reproducible capillary zone electrophoresis method was developed to separate and identify a series of nine peptides of defence interest: bradykinin, bradykinin fragment 1-5, substance P, (arg E8)-vasopressin, luteinizing hormone releasing hormone, bombesin, leucine enkephalin, methionine enkephalin, and oxytocin. Using a 50 mu m x 47 cm capillary column, 22.5 kV separation voltage and a 100 mM pH 2.5 phosphate buffer, all nine peptide could separated in under 10 minutes. Three strategies, which could be used in a fully automated field detection and identification system, were demonstrated for the identification of unknown peptides: comparison of migration times, comparison of electrophoretic mobilities, and co-injection of multiple reference standards. These experiments demonstrate that a separation based analytical method such as capillary electrophoresis could form the basis of a generic detection system for mid-spectrum protein and peptide toxins.
Keywords
Capillary electrophoresis (CE);Toxins;Substance P;Luteinizing hormone releasing hormone;Bomesin;Leucine enkephalin;Methionine enkephalin
Report Number
DRES-M-1463 — Memorandum
Date of publication
01 Apr 1995
Number of Pages
24
DSTKIM No
95-02733
CANDIS No
151936
Format(s):
Document Image stored on Optical Disk;Hardcopy

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