ISOTOPE ENRICHMENT OF PALMITIC ACID ESTERS BY EI GC/MS

PDF

Authors
  1. Zamecnik, J.
  2. Fayolle, C.
  3. Vallerand, A.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
It is well-known that protonation of the molecule (self-chemical ionization) can occur in EI ion source at high analyte concentrations, and is both concentration- and compound-dependent. It is also influenced by the construction and cleanliness of the ion source, and by the presence of other co-eluting compounds in the ion source. Thus, there are several potential sources of variability, which can limit the usefulness of molecular ions for the ion ratio determination. We report here, what we believe to be a simple solution to the problem, namely, the use of mass fragments containing the 1-C(13) label, as suggested by Kienle and Magni. In study (1-C(13)palmitic and metabolism, we have utilized pentafluorobenzyl esters (PFB) of palmitic acid and heptadecanoic acid. These yield positive ion fragments suitable for the calculations of C(13) enrichment.
Keywords
C13 enrichment;EI ion source;Fragments
Report Number
DCIEM-94-21 — Reprint
Date of publication
03 Aug 1994
Number of Pages
2
Reprinted from
Biological Mass Spectrometry, vol 23, 1994, p 804-805
DSTKIM No
95-02732
CANDIS No
151939
Format(s):
Document Image stored on Optical Disk;Hardcopy

Permanent link

Document 1 of 1

Date modified: