IN VITRO OXIME-INDUCED REACTIVATION OF VARIOUS MOLECULAR FORMS OF SOMAN-INHIBITED ACETYLCHOLINESTERASE IN STRIATED MUSCLE FROM RAT, MONKEY AND HUMAN

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Authors
  1. Clement, J.G.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The purpose of this study was to compare the in vitro reactivation of the various molecular forms of soman-inhibited acetylcholinesterase by oximes such as HI-6, toxogonin and PAM, in striated muscle tissue from three species-rat, monkey and human. To simulate the various in vivo conditions the oxime was present either 5 min before and after (Pre-Post) or 5 min after (Post) exposure to the nerve agent soman. In the Pre-Post mode the oxime effects would result from a combinaiton of not only shielding of acetylcholinesterase from soman inhibition by also from immediate reactivation of soman-inhibited acetylcholinesterase. In the Post experimental group the increase in soman-inhibited acetylcholinesterase activity was due to reactivation. HI-6 (Pre-Post) increased significantly the activity of soman-inhibited acetycholinesterase in the rat, human and monkey muscle. HI-6 (Post) was a highly effective reactivator of soman-inhibited acetycholinesterase in the rat muscle and moderately so in the human and monkey muscle. Toxogonin (Pre-Post) and toxogonin (Post) were effective in increasing soman-inhibited acetylcholinesterase activity in rate muscle by were relatively ineffective in the human and monkey muscle. PAM (Pre-Post) and PAM (Post) were ineffective in increasing soman-inhibited acetylcholinesterse activity in muscle from all species examined. TRUNICATED
Keywords
Soman
Date of publication
02 May 1994
Number of Pages
8
Reprinted from
Archives of Toxicology, vol 68, 1994, p 648-655
DSTKIM No
95-03472
CANDIS No
152703
Format(s):
Document Image stored on Optical Disk;Hardcopy

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