IDENTIFICATION OF ILE-SER-BRADYKININ IN AQUEOUS SAMPLES BY LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET AND ELECTROSPRAY MASS SPECTROMETRIC DETECTION

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Authors
  1. Hancock, J.R.
  2. D'Agostino, P.A.
  3. Provost, L.R.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A bioactive peptide, Ile-Ser-Bradykinin, was selected to evaluate sample handling and analysis methods for the detection and identification of mid-spectrum agents in aqueous samples. Ile-Ser-Bradykinin was spiked at the 0.05mg/mL level into distilled water, slough water and water produced by the Large Volume Air Sampler (LVAS), proposed for the Canadian Integrated Biological Agents Detection System (CIBADS). Spiked samples and standards were filtered and analysed at several intervals over a 28 day period to simulate collection and subsequent collection and subsequent analysis of aqueous field samples for mid-spectrum agents. In order to recover Ile-Ser-Bradykinin from these samples, it was necessary to add 0.1% trifluoroacetic acid to the sample prior to filtration through a Millipore Millex HV4 filter, Ile-Ser-Bradykinin was readily detected and identified by liquid chromatography (LC) with ultraviolet (UV) and electrospray mass spectrometric (ESI-MS) detection in all the water samples during the first day, but had degraded to a significant extent within eight days in samples kept at room temperature. When stored at -18C Ile-Ser-Bradykinin standards showed no signs of degradation, even after 28 days. A sample detection of 3 ng (2.5 pmole) was estimated for Ile-Ser-Bradykinin under LC-ESI-MS conditions.
Keywords
Ile Ser Bradykinin;Ultraviolet and Electropspray Mass Spectometric Detection;Toxic peptides;Toxic proteins;Mid-spectrum agents;HPLC;Peptide mapping;Peptide sequencing
Report Number
DRES-M-1465 — Memorandum
Date of publication
01 Jun 1995
Number of Pages
28
DSTKIM No
95-03746
CANDIS No
152846
Format(s):
Document Image stored on Optical Disk;Hardcopy

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