DEVELOPMENT OF ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISAS) TO ANTHRAX FOR THE PERSIAN GULF

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Authors
  1. Nagata, L.P.
  2. Schmaltz, F.L.
  3. Balogh, C.
  4. Bhatti, A.R.
  5. Cherwonogrodzky, J.W.
  6. Schofield, Lawerence N.
  7. Gannon, Victor
  8. Fulton, Elaine
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The report details the research that went into the bacterial component of the enzyme-based immunoassays developed for the Mobile Agent Identification Unit (MAGIDU), and were deployed during Operation Friction in the Persian Gulf in 1991. A rapid whole cell enzyme-linked immunosorbent assay (ELISA) was quickly developed for the identification of selected bacterial agents. The early research concentrated on the identification of Bacillus anthracis whole cells, and the resulting assays were fielded in the Persian Gulf. Anthrax could be reliably detected in 5.5 hrs at concentrations as low as 4.6 x E5 cells/mL (2 mu g/mL). An assay with shortened incubation times was later developed (assay run time of 3.0 - 3.5 hr) with a sensitivity of detection 1.2 x E6 cells/mL (5 mu g/mL). Technical details in the development of these assays are discussed, as well as recommendations for future work.
Keywords
Mobile Agent Identification Unit (MAGIDU)
Report Number
DRES-623 —
Date of publication
01 Jul 1995
Number of Pages
37
DSTKIM No
95-03566
CANDIS No
153036
Format(s):
Document Image stored on Optical Disk;Hardcopy

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