TOXICITY OF SULPHUR MUSTARD IN ADULT RAT LUNG ORGAN CULTURE

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Authors
  1. Sawyer, T.W.
  2. Wilde, P.E.
  3. Rice, P.
  4. Weiss, M.T.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The toxicity of chemical warfare agent sulphur mustard, (bis-(2-chloroethyl)sulphide, HD), was examined in adult rat lung organ cultures. Assessment of HD-induced damage by the MTT cytotoxicity assay indicated that the median lethal concentration (LC50) of HD in these cultures was reproducible, and in the mu M range. Damage to the lung slices was expressed only after a latent period of 48 h and did not increase significantly with longer expression times. Histopathological examination of HD-treated lung cultures showed that the structural changes in the lung tissue paralleled the toxicity measured biochemically, and were also similar to the damage found in animals and man exposed to HD in vivo. This in vitro model offers a useful tool with which to study the toxicity and mechanism of action of sulphur mustard.
Keywords
Histopathology;HD agent;Inhalation
Date of publication
04 Jan 1995
Number of Pages
11
Reprinted from
Toxicology, no 100, 1995, p 39-49
DSTKIM No
96-00745
CANDIS No
153962
Format(s):
Document Image stored on Optical Disk;Hardcopy

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