MODIFICATION OF MITROGEN-INDUCED PROLIFERATION OF MURINE SPLENIC LYMPHOCYTES BY IN VITRO TOCOPHEROL

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Authors
  1. Roy, R.M.
  2. Petrella, M.
  3. Ross, W.M.
Corporate Authors
Concordia Univ, Montreal QUE (CAN) Dept of Biology;Defence Research Establishment Ottawa, Ottawa ONT (CAN)
Abstract
The effect of alpha-tocopherol on in vitro proliferation of murine splenic lymphocyte cultrues supplemented with various concentrations of teh vitamin has been measured at sub-optimal, optimal and supra-optimal levels of the T-cell mitogen Concanavalin A (Con A). In the concentration range (1-25 mug/m1), tocopherol enhanced proliferation when administered up to 24 hours after exposure to sub-optimal and optimal concentrations of Con A: however, at supra-optimal levels of the mitogen, it appeared to inhibit proliferation. In the concentration range 50-100 mug/m1, tocopherol supplementation only enhanced proliferation in response to sub-optimal concentration of Con A. The spontaneous proliferation of lymphocytes in the absence of mitogens was increased by tocopherol supplementation at all concentrations tested. In contrast, there appeared to be only slight stimulation of B-cell proliferation in response to optimal concentration of bacterial lipopolysaccharide (LPS) by lower levels of vitamin E. Tocopherol supplementation of cultures over a broad range of concentrations (0.5 - 100 mug/m1) had no significant effect on cell viability before onset of proliferation at 18 hours after exposure to Con A, nor was there evidence of earlier onset of DNA synthesis in response to mitogen in the presence of 5 mug/m1 of the vitamin. TRUNCATED
Keywords
Interleukins;Mitogens;Immune response
Date of publication
01 Jan 1991
Number of Pages
20
Reprinted from
Immunopharamacology and Immunotoxicology, vol 13, no 4, 1991, p 531-550
DSTKIM No
96-02597
CANDIS No
157124
Format(s):
Document Image stored on Optical Disk;Hardcopy

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