A TURBIDO-HAEMOLYTIC ASSAY FOR COMPLEMENT

Authors
  1. DiNinno, V.L.
  2. Chenier, V.K.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A method for the haemolytic assay of complement is described. The method is based on the turbidimetric quantitation of sheep red blood cells at a wavelength of 640 nm. The approach allows for the continuous and simultaneous assessment of complement activity and results in a substantial reduction in the time required to perform complement assays as opposed to the conventional haemolytic assays. The turbido-haemolytic assay is used to investigate the anti-complementary activity of kappa and lambda carrageenans. Lambda carrageenan, the more sulphated polymer, is the more potent inhibitor of complement activation. The application of the technique to the detection and/or identification of microorganisms is presently being investigated.
Report Number
M-1009 —
Date of publication
15 Feb 1981
Number of Pages
19
DSTKIM No
82-00205
CANDIS No
34908
Format(s):
Hardcopy;Originator's fiche received by DSIS

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