PARTIAL CHARACTERIZATION OF SMALL PLASMID DEOXYRIBONUCLEIC ACID PRESENT IN NEISSERIA MENINGITIDIS

Authors
  1. Bhatti, A.R.
  2. O'Hara, K.
  3. White, L.A.
  4. Bryan, L.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The presence of plasmid DNA in Neisseria meningitidis was investigated. N. meningitidis isolates from clinical meningitis and highly virulent in mice, were found to harbour a small plasmid. Agarose gel electrophoresis of crude lysates from different strains revealed a single plasmid DNA species with the same apparent electrophoretic mobility. Using agarose gel electrophoresis its molecular weight was calculated as 3.4 megadaltons. Electron microscopic observation revealed that plasmid DNA molecules were predominently in the covalently closed form. The estimated contour length of the open circular form was 1.6 mu m. The function of the plasmid DNA is presently unknown.
Report Number
295 —
Date of publication
15 May 1981
Number of Pages
15
DSTKIM No
82-00208
CANDIS No
34911
Format(s):
Hardcopy;Originator's fiche received by DSIS

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