INTRACELLULAR NATURE OF THE ORTHOPHOSPHATE ACCUMULATED BY AEROBACTER AEROGENES

Authors
  1. White, L.A.
  2. MacLeod, R.A.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Phosphate uptake and accumulation by A. aerogenes was shown to be energy dependent but to follow different paths depending on the energy source provided for growth. In cells respiring on glucose, inorganic orthophosphate was shown to enter the cell as a nucleotide triphosphate (ATP or UTP) and to be immediately transesterified into intermediates of the glycolytic (phosphoenolpyruvate) and pentose phosphate (6-phosphogluconate) pathways. No evidence was obtained for an inorganic orthophosphate "pool". When succinate was provided as the energy source, the phosphate was also transported as ATP but passed into a "pool" prior to entry into other metabolites.
Keywords
Aerobacter aerogenes
Report Number
324 —
Date of publication
15 Sep 1981
Number of Pages
27
DSTKIM No
82-00649
CANDIS No
35306
Format(s):
Hardcopy;Originator's fiche received by DSIS

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