X-RAY MICROANALYSIS OF FLUID SPACES IN THE FROZEN COCHLEA

Authors
  1. Hunter-Duvar, I.
  2. Landolt, J.
  3. Cameron, R.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
The cochlea is quick-frozen and then opened while under liquid nitrogen to expose the scalae of all turns in the mid-modiolar section. Still under liquid nitrogen, the cochlea is transferred to the cold stage of a pre-vacuum chamber especially constructed for attachment to the scanning electron microscope. Under partial vacuum, it is moved to the cold stage in the high vacuum chamber of a field-emission scanning electron microscope. Energy dispersive X-ray analysis demonstrates the relative levels of sodium, potassium, and chlorine in the fluid spaces of the cochlea.
Report Number
RP-80-P-35 — Paper 80P35 presented at the 17th Workshop on Inner Ear Biology, Stockholm, June 23-25, 1980
Date of publication
01 Jan 1981
Number of Pages
5
Reprinted from
Arch Otorhinolaryngol, vol 230, 1981, p 245-249
DSTKIM No
82-03211
CANDIS No
37584
Format(s):
Hardcopy;Originator's fiche received by DSIS

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