THE MECHANISM OF BINDING AND SECRETION OF AKALINE PHOSPHATASE ACROSS THE CELL MEMBRANES OF PSEUDOMONAS AERUGINOSA

Authors
  1. Bhatti, A.R.
  2. Ingram, J.M.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);McGill Univ, Ste-Anne-de-Bellevue QUE (CAN) Dept of Microbiology
Abstract
During growth, pH of the medium decreases resulting in the inactivation of cell-free and surface-bound alkaline phosphatase, whereas periplasm-located alkaline phosphatase is not affected. The decreased pH of the medium induced permeability changes in the outer cell wall which resulted in the complete release of alkaline phosphatase after sucrose extraction. The permeability change of the outer cell wall is pH dependent and reversible. The periplasm-located alkaline phosphatase remains constant during growth of Pseudomonas aeruginosa. A hypothetical model of the mechanism for the release of periplasm-located proteins into the growth medium during growth is presented.
Report Number
M-1068 —
Date of publication
15 Nov 1982
Number of Pages
13
DSTKIM No
83-00208
CANDIS No
38365
Format(s):
Hardcopy;Originator's fiche received by DSIS

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