RETICULO-FIBROBLASTOID STROMAL CELL PROGENITORS (CFU-RF) IN MURINE BONE MARROW

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Authors
  1. Ross, W.M.
  2. Peeke, J.
  3. Hockey, A.J.
  4. Polyak, S.
  5. Izaguirre, C.A.
Corporate Authors
Defence Research Establishment Ottawa, Ottawa ONT (CAN)
Abstract
The hemopoietic inductive microenvironment (HIM) of the bone marrow is responsible for secretion of growth factors that regulate hemopoiesis. It is composed of an extracellular matrix and a complex variety of cell types with a range of functions related to blood cell development. In order to understand how such a complex system operates, it will first be necessary to determine the role(s) of the integral parts. Several of the stromal cell types have been identified morphologically in various culture systems, and some of their functions have been elucidated. This document reports the identification of a new stromal cell type in mouse bone marrow that appears similar if not identical to its human counterpart. When bone marrow cells are placed in methylcellulose/plasma clot culture with phytohemagglutinin-stimulated human leukocyte-conditioned medium in the presence of bovine calf serum (BCS), mercaptoethanol, and hydrocortisone, extensive branching colonies develop within 14 days.
Keywords
Stromal cells;Adipocytes;Stem cells
Date of publication
31 Oct 1990
Number of Pages
6
Reprinted from
Exp Hematol, vol 19, 1991, p 1069-1074
DSTKIM No
96-02600
CANDIS No
394282
Format(s):
Document Image stored on Optical Disk;Hardcopy

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