RETICULO-FIBROBLASTOID STROMAL CELL PROGENITORS (CFU-RF) IN MURINE BONE MARROW
- Authors
- Corporate Authors
- Defence Research Establishment Ottawa, Ottawa ONT (CAN)
- Abstract
- The hemopoietic inductive microenvironment (HIM) of the bone marrow is responsible for secretion of growth factors that regulate hemopoiesis. It is composed of an extracellular matrix and a complex variety of cell types with a range of functions related to blood cell development. In order to understand how such a complex system operates, it will first be necessary to determine the role(s) of the integral parts. Several of the stromal cell types have been identified morphologically in various culture systems, and some of their functions have been elucidated. This document reports the identification of a new stromal cell type in mouse bone marrow that appears similar if not identical to its human counterpart. When bone marrow cells are placed in methylcellulose/plasma clot culture with phytohemagglutinin-stimulated human leukocyte-conditioned medium in the presence of bovine calf serum (BCS), mercaptoethanol, and hydrocortisone, extensive branching colonies develop within 14 days.
- Keywords
- Stromal cells;Adipocytes;Stem cells
- Date of publication
- 31 Oct 1990
- Number of Pages
- 6
- Reprinted from
- Exp Hematol, vol 19, 1991, p 1069-1074
- DSTKIM No
- 96-02600
- CANDIS No
- 394282
- Format(s):
- Document Image stored on Optical Disk;Hardcopy
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