RAPID RATE-KINETIC TURBIDOMETRIC ASSAY FOR QUANTITATION OF ANTIVIRAL COMPLEMENT-FIXING ANTIBODIES

Authors
  1. Fulton, R.E.
  2. DiNinno, V.L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A rapid rate-kinetic turbidometric assay for the quantitation of viral complement fixing antibodies has been developed, using adenovirus as a model. The procedure is based on the turbidometric quantitation of intact sheep red blood cells and measures the rate of hemolysis at maximum velocity occurring in the presence of residual complement not fixed by the antigen-antibody reaction. Reagents were standarized and assays performed using a microprocessor-controlled spectrophotometer with thermoregulated cell compartment and kinetic assay capability. Eleven sera were assayed for complement fixing antibodies both by the conventional microtiter technique and by the rapid turbidometric method described here. TRUNCATED
Report Number
M-1141 —
Date of publication
15 Feb 1985
Number of Pages
25
DSTKIM No
85-01335
CANDIS No
46152
Format(s):
Hardcopy;Originator's fiche received by DSIS

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