ATP-HEMOGLOBIN PURIFICATION BY ATP-AGAROSE AFFINITY CHROMATOGRAPHY

Authors
  1. Hsia, C.J.C.
  2. Hronowski, L.J.
  3. Er, S.S.
  4. Persaud, K.
  5. Ansari, M.R.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
The authors have recently demonstrated that ATP-agarose can be used for the purification of stroma-free hemoglobin. In the present communication, the authors show that ATP-modified and ATP-unmodified hemoglobin can be separated by affinity chromatography on ATP-agarose gels. This separation procedure gives ATP-hemoglobin conjugate in greater than 98% purity, based on the removal of unmodified hemoglobin. Furthermore, the purified ATP-hemoglobin is shown to have better oxygen-releasing properties, i.e. lower oxygen affinity, than that of the reaction mixture and the unmodified hemoglobin.
Report Number
DCIEM-86-P-18 — Research Paper
Date of publication
01 Jan 1986
Number of Pages
5
Reprinted from
J of Chromatography, no 381, 1986, p 153-157
DSTKIM No
87-00101
CANDIS No
48954
Format(s):
Hardcopy;Originator's fiche received by DSIS

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