LIPOSOMES AND TEAR FLUID. I. RELEASE OF VESICLE-ENTRAPPED CARBOXYFLUORESCEIN

Authors
  1. Barber, R.F.
  2. Shek, P.N.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN);Toronto Univ, Toronto ONT (CAN) Dept of Clinical Biochemistry
Abstract
The successful application of liposomes as a topical ophthalmic drug delivery device requires knowledge of vesicle stability in the presence of tear fluid. The release of 5-carboxyfluorescein from large unilamellar liposomes in the presence of rabbit tear fluid was studied in vitro as a function of bilayer cholesterol content. Reverse evaporation vesicles were prepared from egg phosphatidylcholine, stearylamine and varying amounts of cholesterol. Both the rate and the extent of fluorescent dye release were significantly increased in the presence of rabbit tear fluid at all cholesterol levels. However, by incorporating increasing amounts of cholesterol in the vesicle bilayers, tear-induced leakage was reduced. The release kinetics reported in this study are similar to those observed in the presence of human serum. TRUNCATED
Report Number
DCIEM-86-P-41 —
Date of publication
15 Jun 1986
Number of Pages
7
Reprinted from
Biochimica et Biophysica Acta, no 879, 1986, p 157-163
DSTKIM No
87-00612
CANDIS No
49460
Format(s):
Hardcopy;Originator's fiche received by DSIS

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