CONSTRUCTION OF A RECOMBINANT VIRAL VECTOR CONTAINING PART OF THE NUCLEOCAPSID PROTEIN GENE OF NEWCASTLE DISEASE VIRUS

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Authors
  1. Bader, D.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
This report describes the procedures used to clone a 673 base pair gene fragment of the major nucleocapsid protein gene of Newcastle disease virus into a viral vector molecule for the purpose of maintaining a stable, long-term, renewable source of this target sequence for gene probe studies. The gene fragment was prepared by reverse-transcription polymerase chain reaction of Newcastle disease virus RNA and was cloned into a viral DNA vector M13mp18 RF to produce a recombinant DNA molecule. The cloned fragment was shown to be present in the recombinant clones based on (I) clonal selection on indicator plates: (ii) restriction enzyme analysis; (iii) gene probe analysis and (iv) nested PCR amplification.
Keywords
Polymerase chain reaction
Report Number
DRES-M-1464 — Memorandum
Date of publication
01 Sep 1995
Number of Pages
33
DSTKIM No
96-02386
CANDIS No
498220
Format(s):
Document Image stored on Optical Disk;Hardcopy

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