A SUSTAINED RELEASE BACTERIAL INOCULUM INFUSION MODEL OF INTRA-ABDOMINAL INFECTION IN CONSCIOUS RATS: BACTERIOLOGY, METABOLISM, AND HISTOPATHOLOGY

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Authors
  1. Martineau, L.
  2. Shek, P.N.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
The report describes the development of a rat peritonitis model that simulates a slow, sustained bacterial release from the gut. Septic animals (SEP) received an intraperitoneal infusion of a bacterial inoculum (6.5 x E8 colony forming units Escherichia coli) over 12 h, while control rats (CON) received a sterile inoculum. This model yielded a 52% mortality over 7 days in SEP, with deaths usually occurring 24-48 h after the onset of infusion. Septic rats showed greater febrile responses and body weight losses than those of CON, as well as mild hyperlactacidemia, hypoglycemia, and episodic bacteremia. Maximum bacterial counts in peritoneal fluid and several organs of SEP were observed at 36 h, with bacterial counts progressively decreasing by 7 days to levels similar to those observed at 12h. Lung and spleen wet weights increased by 17% at 36 h and 35% at 7 days post-infection in SEP. Histological evaluation of random organ samples revealed mild to moderate morphological changes in SEP while CON showed no or minimal changes in the parameters measured during the study. This new model of chronic peritonitis in the rat reproduces many of the clinical features observed in human sepsis, and thus should prove to be a useful tool in further studies of the pathophysiology of peritonitis.
Keywords
Sepsis
Report Number
DCIEM-95-38 — Reprint
Date of publication
01 May 1996
Number of Pages
9
Reprinted from
Shock, vol 5, no 6, 1996, p 446-454
DSTKIM No
96-02536
CANDIS No
499186
Format(s):
Document Image stored on Optical Disk;Hardcopy

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