ANTIBODY-BASED HAND-HELD TEST KITS FOR MONITORING AND IDENTIFICATION OF TOXINS AND BIOLOGICAL WARFARE AGENTS: PROCEDURES FOR NATO PROJECT GROUP 32 PHASE II ROUND ROBIN TRIALS

PDF

Authors
  1. Fulton, R.E.
  2. Stadnyk, L.
  3. Kayfish, C.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
In July 1996, DRES was host to NATO Project Group 32 (Antibody-based Hand-held Test Kits for Monitoring and Identification of Toxins and Biological Warfare Agents) for the development and conduct of round robin trials in which hand-held test kits were evaluated for sensitivity, specificity and reaction time in identification of Staphylococcal Enterotoxin B in samples containing a variety of potential assay interferents. For these trials, DRES developed a battery of potential assay interferents, assay and laboratory protocols for the conduct of round robin tests, and computer programs for the coding and decoding of samples and analysis and reporting of trial data. Trials were conducted in which four test kits were evaluated for identification of Staphylococcal Enterotoxin B in the presence of 14 different potential interferents. A total of 1568 assays were performed and analysed over a period of two days. The methods and procedures developed for these trials and described in this report will serve as guidance in the development of future round robin trials requiring the processing of large numbers of samples in a limited time frame.
Keywords
HHTK (Hand Held Test Kit);Interferents
Report Number
DRES-SP-185 — Special Publication
Date of publication
01 Sep 1996
Number of Pages
142
DSTKIM No
97-02575
CANDIS No
502659
Format(s):
Hardcopy;Document Image stored on Optical Disk

Permanent link

Document 1 of 1

Date modified: