LIQUID CHROMATOGRAPHY CONTINUOUS-FLOW MATRIX-ASSISTED LASER DESORPTION IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY FOR THE DETECTION OF BIOACTIVE PEPTIDES AND PROTEINS

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Authors
  1. Li, L.
  2. D'Agostino, P.A.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Alberta Univ, Edmonton ALTA (CAN) Dept of Chemistry
Abstract
The general approach of combining liquid chromatography (LC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry for on-line detection of peptides and proteins based on the use of a continuous-flow (CF) probe is presented. Recent advances in time-of-flight mass spectrometric instrumentation for CF-MALDI are reported. Using 3-nitrobenzyl alcohol (3NBA) as the liquid matrix, stable flow can be readily achieved and the 3-port interface does not introduce chromatographic degradation for LC-MS. Separation and detection of low picomoles of peptides and proteins can be done with CF-MALDI LC-MS. Parallel ion extraction and time-lag focusing (TLF) are shown to provide enhanced performance with regard to mass resolution. However, mass resolution is generally poor for proteins with masses above approximately 6000 u using 3NBA as the matrix. Strong adduct ion formation with the use of 3-NBA as the liquid matrix is believed to be the main cause of this resolution reduction. It is argued that CF-MALDI is technically viable approach for LC-MS; but the overall performance and wide use of this method depend on the discover of new liquid matrices that are suitable for continuous flow and provide much enhanced utility for MALDI over 3NBA, particularly for proteins. TRUNCATED
Report Number
DRES-CR-97-12 — Contractor Report
Date of publication
01 Jun 1997
Number of Pages
28
DSTKIM No
97-03486
CANDIS No
503630
Format(s):
Hardcopy;Document Image stored on Optical Disk

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