RADIATION DAMAGE AND IMMUNE SUPPRESSION IN SPLENIC MONONUCLEAR CELL POPULATIONS

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Authors
  1. Harrington, N.P.
  2. Chambers, K.A.
  3. Ross, W.M.
  4. Filion, L.G.
Corporate Authors
Defence Research Establishment Ottawa, Ottawa ONT (CAN);Ottawa Univ, Ottawa ONT (CAN) Dept of Physiology
Abstract
We have examined alterations in all of the major splenic mononuclear cell (SMNC) population in C57B1/6 mice following whole-body irradiation (0-700 cGy) in order to determine which populations may play a role in active immune suppression and/or haematopoietic recovery. A protocol has been established for characterization and differentiation by flow cytometric analysis (FCA) of the major MNC population in the mouse spleen: T lymphocytes (CD4+ and CD8+ cells), B lymphocytes, natural killer (NK) cells, and monocytes/macrophages. Ionizing radiation caused decreased spleen cellularity and decreased ability of surviving SMNC to respond to mitogen. FCA revealed alterations in the relative composition of the constituent splenic cell populations following irradiation, reflecting differential radiosensitivity, with selective enrichment of NK cells (seven-fold) and CD4+ T lymphocytes (three-fold). Enrichment developed during the 7-day post-irradiation period. In addition, some MNC became activated in a dose- and time-dependent fashion following whole-body irradiation, as indicated by expression of CD71, the transferrin receptor. These cells were CD34+ and Thy 1.2+, but were CD4- or CD8- as well as CD45- (B cell). The observed increase in NK cells corresponds with a previously reported increase in natural suppressor (NS) cells following total-lymphoid irradiation (TLI). TRUNCATED
Keywords
Flow cytometry;Radiosensitivity;Immune suppression;Immune system;Natural killer cells
Date of publication
09 Oct 1996
Number of Pages
8
Reprinted from
Clin Exp Immunol, no 107, 1997, p 417-424
DSTKIM No
98-00031
CANDIS No
506720
Format(s):
Hardcopy;Document Image stored on Optical Disk

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