ELECTROPHORETIC SEPARATION OF BIOLOGICAL MOLECULES ON MICRO-MACHINED SILICA PLATES (FINAL REPORT)

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Authors
  1. Harrison, J.
  2. Lee, W.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Alberta Univ, Edmonton ALTA (CAN) Dept of Chemistry
Abstract
The principal goals of this project during the funding period were to demonstrate the separation of the products of immunological reactions and of DNA binding assays on a microchip. After achieving this result we were to integrate one of these two classes of reaction onto a chip, along with the separation itself. Electroosmotic flow was to be used to control the fluid flow and pumping within the chip. The choice of which reaction to demonstrate was to be made at the end of the first year, largely based on the success with each type of separation at that time. The report below describes our results. As a brief summary we were able to demonstrate immunoassay separations of the biological warfare simulant phage virus MS-2, and of small molecule antigens used as drugs, specifically theophyliline. Separation of DNA samples obtained from BW simulants, following reaction with a fluorescent probe molecule, was also shown. On-chip reactions and separations were demonstrated for the immunological determination of theophylline. Our results demonstrate that electrokinetic pumping and control of immuno and DNA assay reagents is possible within a microchip. The quality of results was high, indicating the device could be used in principle for automated BW agent determination. TRUNCATED
Keywords
Immunoassay separations
Report Number
DRES-CR-97-19 — Contractor Report
Date of publication
01 Jun 1997
Number of Pages
11
DSTKIM No
98-00342
CANDIS No
506936
Format(s):
Hardcopy;Document Image stored on Optical Disk

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