QUANTITATION OF EPINEPHRINE, NOREPINEPHRINE, DOPAMINE, METANEPHRINE AND NORMETANEPHRINE IN HUMAN PLASMA USING NEGATIVE ION CHEMICAL IONIZATION GC-MS

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Authors
  1. Zamecnik, J.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
Quantitation of epinephrine (EPI), norepinephrine (NEPI), dopamine (DOP), metanephrine (MET) and normetanephrine (NMET) in human blood plasma by gas chromatography-mass spectrometry with electron capture negative ion chemical ionization (EC-NCI) using a 5% ammonia/methane mixture as a reagent gas is described. Deuterium labeled D3-epinephrine, D3-norepinephrine and D2-dopamine were used as internal standards. D2-epinephrine was used for quantitation of the MET and NMET. Plasma samples were extracted using ion exchange solid phase tubes, freeze dried and derivatized with pentafluoropropionic acid anhydride. The quantitation ions used for the selective ion monitoring were: EPI - m/z 767, D3-EPI - m/z 770, NEPI - m/z 753, D3-NEPI - m/z 756, DOP - m/z 571, D2-DOP - m/z 573, MET - m/z 635 and NMET - m/z 621. The assay sensitivity was sufficient to measure resting concentrations of these biogenic amines in 0.1 mL of human blood plasma (the mean measured concentrations were: EPI - 25 pg/mL; NEPI - 280 pg/mL; DOP - 33 pg/mL; MET - 28 pg/mL; NMET - 53 pg/mL). The method was used to analylze over 1000 specimens and proved to be rugged, specific and reproducible.
Keywords
Electron Capture Negative ion Chemical Ionization
Report Number
DCIEM-97-P-63 — Paper
Date of publication
07 Apr 1997
Number of Pages
8
Reprinted from
Journal of Analytical Sciences and Spectroscopy, 1997, p 106-112
DSTKIM No
98-00322
CANDIS No
506958
Format(s):
Hardcopy;Document Image stored on Optical Disk

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