DETERMINATION OF BIOACTIVE PEPTIDE MOLECULAR MASS USING ELECTROSPRAY AND MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY

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Authors
  1. D'Agostino, P.A.
  2. Hancock, J.R.
  3. Provost, L.R.
  4. Tornes, J.A.
  5. Dai, Y.
  6. Li, L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Twelve bioactive peptides, ranging in molecular masses from 600 and 4500 u, were selected for ESI-MS and MALDI-TOF-MS analysis in order to assess the spectrometric data that could be accessed for rapid chemical/biological warfare agent screening purposes. Monoisotopic molecular mass data were obtained for all the peptides by ESI-MS with a tri-focusing magnetic sector instrument in the continuum mode using resolutions between 3000 and 6000 (10% valley definition). MALDI-TOF-MS data were collected with an instrument that featured a four-plate source design, pulsed ion extraction for time-lag focusing, and a 1-m linear flight tube. High resolution mass measurements with the tri-focusing magnetic sector instrument were the most accurate. Errors between theoretical and calculated monoisotopic (or average) molecular mass were in the 0.3 to 18 ppm and 10 to 96 ppm range during ESI-MS and MALDI-TOF-MS analysis, respectively. ESI-MS data may be acquired with as little as 1 to 10 pmoles of peptide at resolutions of 2000 to 3000 (10% valley definition) with the tri-focusing magnetic sector instrument. The MALDI-TOF-MS instrument described offered improved sensitivities with only 0.1 to 1 pmole of peptide being required. In both cases analyses times were rapid, with analyses taking 2 to 5 minutes per sample. Both ionization techniques, ESI and MALDI, would be suitable for rapid molecular mass screening purposes.
Keywords
Matrix Assisted Laser Desorption Ionization (MALDI);Electrospray;Bioactive peptides;Toxic peptides
Report Number
DRES-M-1497 — Memorandum
Date of publication
01 Jan 1998
Number of Pages
25
DSTKIM No
98-00454
CANDIS No
507091
Format(s):
Hardcopy;Document Image stored on Optical Disk

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