MOLECULAR CLONING AND SEQUENCING OF THE 26S REGION OF WESTERN EQUINE ENCEPHALITIS VIRUS STRAIN 71V-1658

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Authors
  1. Schmaltz, F.L.
  2. Nagata, L.P.
  3. Rayner, G.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A 3100 bp cDNA clone (pcDW-12) of the 26S region of western equine encephalitis (WEE) virus strain 71V-1658 was identified by dot blot hybridization from a cDNA library. The missing 5' end was PCR-amplified and engineered into pcDW-12 to obtained a full ength clone of the 26S region. The resulting construct (XH-7) was restriction mapped and completely sequenced on both strands. Only eleven of the sixty-three nucleotide differences resulted in amino acid changes when the sequence was compared to WEE strain BFS-1703 (Hahn et al, 1988). In addition, the high degree of conservation of the structural proteins was maintained when compared to the N-terminal sequence of the E1 and E2 proteins of the McMillan strain of WEE (Bell et al, 1983). The conserved nature of the structural proteins would indicate that one strain of WEE should be able to cross-protect against all WEE strains. A 2.2 kb fragment at the 5' end of the genome was also cloned and sequenced, and demonstrated high homology to the available sequences for eastern equine encephalitis (EEE) and Highlands J (HJ) viruses, adding fruther evidence that the netire 5' nonstructural region of WEE was derived from EEE. In summary, the genetic cloning and sequencing of the WEE 26S region marks the critical first step in the generation of a subunit vaccine to WEE.
Keywords
Biological strains;WEEV (Western Equine Encephalitis Virus);Alphaviruses;Sequencing;Subunit vaccines
Report Number
DRES-633 —
Date of publication
01 Jan 1997
Number of Pages
66
DSTKIM No
98-00647
CANDIS No
507558
Format(s):
Hardcopy;Document Image stored on Optical Disk

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