BACKGROUND AEROSOL CHARACTERISTICS MEASURED WITH A FLUORESCENCE AERODYNAMIC PARTICLE SIZER: SENSITIVITY OF FLAPS PERFORMANCE

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Authors
  1. Ho, J.
  2. Spence, M.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
From observations done with flow cytometry, it was found that a single spore in liquid stream could be induced to fluoresce at 340-60 nm. In a prototype instrument, similar results were obtained when spore particles in air were excited with a ultraviolet (UV) laser. The Fluorescence Aerodynamic Particle Sizer (FLAPS) measures particle size as well as intrinsic fluorescence characteristics of individual particles in an aerosol stream. Laser light at UV wavelength is used to selectively excite aerosol material of a desired size range. The measured fluorescence signals represent intrinsic biological properties of the particles. By this method, it has been shown that inert particles like sand can be distinguished from biological particles like spores and vegetative bacteria. This instrument and its prototype have been demonstrated to effectively detect and characterise biological aerosols during joint field trial exercises in both 1995 and 1996. Integral with the FLAPS design is an aerosol concentrator that concentrates particles in air about 400-500 times normal ambient concentration. This makes it possible to sample particles at a rate of 3 seconds and within this period, about 300-3000 total particles are processed for valid statistical counts. TRUNCATED
Keywords
Bio-aerosol detection;Fluorescent Aerodynamic Particle Sizer;FLAPS;Fluorescence of Biological Aerosols
Report Number
DRES-M-1504 — Memorandum
Date of publication
01 Mar 1998
Number of Pages
22
DSTKIM No
98-00907
CANDIS No
507742
Format(s):
Hardcopy;Document Image stored on Optical Disk

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